Accession Data

MD124 Data Provided By Dorothy Watson

The following data was provided by Dorothy Watson on a new accession (MD124) donated to INVAM at the beginning of October, 1996. It exemplifies the kinds of information needed on an accession to begin a thorough characterization that merges culture behavior and ecological properties.

Local reference: Glomus macrocarpum Ith-24MD (= Ithaca isolate #24, from Maryland)

Collector: Dorothy Watson

Last known address: Department of Soil, Crop and Atmospheric Sciences, Cornell University, Ithaca, NY 14853, USA

Collection date of original sample: April, 1995

Most detailed locality of sampling: Plot # 2: The location (in Maryland State Plane Meters) = 408690.535 Fasting and 151267.474 Northing.

Next most detailed locality information: Long-Term Cropping System Field Trials on a 40 acre field site located at the USDA Beltsville Agricultural Research Center in Beltsville, MD

County: Prince George’s

State: Maryland

Country: USA

Continent: North America

Biome

Agricultural field in production for 60 years, the first 50 years in animal forage and pasture; from 1985 to 1992 in alfalfa, cut for hay and managed with conventional fertilizers and pesticides; from 1993 to 1994 in no till corn, managed with conventional fertilizers and pesticides.

Latitude: 390143.64635

Longitude: 765400.96101

Altitude: 115 It (34.5 meters)

Edaphic Measurements

Keyport silt loam variant, an Aeric Ochraqunlt, clayey, mixed, mesic, somewhat poorly drained.

P: 200 kg/ha

OM%: 1.72g/lOOg total C

Texture: clayey siltloam

Any otherions: Total N: 0.13g/lOOg

pH: 6.7 (water extract)

First Culture of Fungal Isolate

Start date: May 9th, 1995

Sample date: October 2nd, 1995

Host: sorghum-sudangrass hybrid

Substrate: 1:1 (v/v) mixture of coarse sand to pasteurized field soil (pH = 6.9).

Type of culture: set up with five similar looking spores collected directly from site #2 field soil (collected in April)

Details: The pot used was an 8 inch conetainer.

Final Culture

Start date: January 12th, 1996 (after the pot culture material had been stored air-dry and cool for 2 months)

Sample date: August 28th. 1996

Host: Corn (Zea mays)

Substrate: 1:1 mixture of coarse sand to pasteurized field soil

Type of culture: 25 g of whole inoculum from the original culture was added in a layer about 4 inches under the soil surface, in a deepot. The remaining potting mixture was a 1:1 (v/v) mix of coarse sand and pasteurized soil (pH = 6.9).