Septoglomus deserticola


(reference accession CA113)


Whole Spores

Orange brown dark to pale various sizes spheres

Orange brown spheres shiny translucent

COLOR: orange-brown to dark red-brown. Most are a reddish-brown (20/80/100/0).

SHAPE: Globose to subglobose usually, but sometimes irregular (see right photo above).

SIZE DISTRIBUTION: Highly variable, from 60-140 µm. Spores typically are formed on very robust hyphae around the root, so they aren’t dislodged as easily as many other glomoid species.


Subcellular Structure of Spores

SPORE WALL: Two layers (L1, L2), with the outer layer adherent until it degrades and sloughs.

L1: A hyaline layer; 2-4 µm thick; does not react in Melzer’s reagent. In aged spores, this layer is almost always missing.

L2: An yellow-brown to orange-brown laminate layer, 4-6 µm thick, which is continuous with the inner layer of a persistent subtending hypha. In Melzer’s reagent, this layer produces a dextrinoid reaction (appearing darker red-brown)

 

In PVLGIn Melzer's Reagent

pvlg slide spore L1 and L2 darker edge

Melzers subtending hypha stubby branch from spore layers


Subtending Hypha

COLOR: Cylindrical to slightly flared.

WIDTH: 8-12 µm at base of spore.

WALL STRUCTURE: Two layers (L1, L2) that are continuous with the two layers of the spore wall. The outer hyaline layer (L1) is very thin (1 µm or less). The inner layer (L2) is concolorous with the same layer in the spore wall, 1-3 µm thick at the spore base and then thinning to < 2 µm. The hyphae do not break easily, so that spores often are clustered together very loosely (see photo) or are closely associated with root fragments.

 

Deserticola

4 clustered smashed spores with hyphae melzers

Occlusion hyphae and irregular oval


OCCLUSION: Usually a septum.


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High Resolution Images

orange brown dark to pale various sizes spheres
Spores
Darker brown spore
In PVLG
pvlg slide spore L1 and L2 darker edge
In PVLG
Melzers subtending hypha stubby branch from spore layers
In Melzer's reagent

Links to Gene Sequences in Genbank

rRNA genes

beta-tubulin gene